Use este identificador para citar ou linkar para este item: http://higia.imip.org.br/handle/123456789/702
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dc.contributor.authorAvelino, Karen Yasmim Pereira dos Santos-
dc.contributor.authorOliveira, Léony Soares de-
dc.contributor.authorLucena-Silva, Norma-
dc.contributor.authorAndrade, ‪Cesar Augusto Souza de-
dc.contributor.authorOliveira, Maria Danielly Lima de-
dc.date.accessioned2022-06-07T15:57:02Z-
dc.date.available2022-06-07T15:57:02Z-
dc.date.issued2021-
dc.identifier.urihttp://higia.imip.org.br/handle/123456789/702-
dc.description.abstractConsidering the low sensitivity of cytological exams and high costs of the molecular methods, the development of diagnostic tests for effective diagnosis of HPV infections is a priority. In this work, biosensor composed of polypyrrole (PPy) films and gold nanoparticles (AuNPs) was obtained for specific detection of HPV genotypes. The biosensor was developed by using flexible electrodes based on polyethylene terephthalate (PET) strips coated with indium tin oxide (ITO). Polymeric films and AuNPs were obtained by electrosynthesis. Oligonucleotides sequences modified with functional amino groups were designed to recognize HPV gene families strictly. The modified oligonucleotides were chemically immobilized on the nanostructured platform. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for the analysis of the electrode modification and monitoring of molecular hybridization. Electrochemical changes were observed after exposure of the bio-sensors to plasmid samples and cervical specimens. The biosensor based on the BSH16 probe showed a linear concentration range for target HPV16 gene detection of 100 pg μL.pt_BR
dc.language.isoenpt_BR
dc.subjectPapillomaviridaept_BR
dc.subjectNanopartículaspt_BR
dc.subjectGenespt_BR
dc.titleFlexible sensor based on conducting polymer and gold nanoparticles for electrochemical screening of HPV families in cervical specimenspt_BR
dc.higia.programArtigos científicos colaboradores IMIPpt_BR
dc.higia.tipoArtigo Científicopt_BR
dc.higia.pages9 p.pt_BR
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